Anti-SHP1 (phospho Y536) antibody (ab51171)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho Y536) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho Y536) -
Host species
Rabbit -
Specificity
ab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51171 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y536) antibody (ab51171)ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-SHP1 (phospho Y536) antibody (ab51171)Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes) Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)
