Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)
![Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-447668-anti-ataxin7antibody-epr2413689-western-blot-lysate.jpg "Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24136-89] to Ataxin 7
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Ataxin 7 antibody [EPR24136-89]
See all Ataxin 7 primary antibodies -
Description
Rabbit monoclonal [EPR24136-89] to Ataxin 7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain tissue lysate; Neuro-2a whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24136-89 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)All lanes : Anti-Ataxin 7 antibody [EPR24136-89] (ab259829) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The MW is consistent to literature (PMID:22367614).
Negative control: mouse kidney (PMID:11099453).
Exposure time: 81 seconds.
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![Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-447669-anti-ataxin7antibody-epr2413689-western-blot-neuroblast.jpg)
Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)Anti-Ataxin 7 antibody [EPR24136-89] (ab259829) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
WB positive cell line: Neuro-2a (PMID:25306109)
Exposure time: 125 seconds.
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![Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-448067-benefitsofrecombinantantibodies.png)
Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)