Human EEA1 knockout HeLa cell lysate (ab256897)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261993 - Human EEA1 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human EEA1 knockout HeLa cell lysate (ab256897)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: EEA1 knockout HeLa cell lysate (20µg)
Lane 3: Daudi cell lysate (20µg)
Lane 4: SH-SY5Y cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab109110 observed at 175 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab109110 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker was shown to specifically react with EEA1 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab261822 (knockout cell lysate ab256897) lane below 175kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and EEA1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109110 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human EEA1 knockout HeLa cell lysate (ab256897)Homozygous: 1 bp insertion in exon13