Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free
    See all Coilin primary antibodies

  • Description

    Rabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and HeLa cell lysates.

  • General notes

    ab249972 is the carrier-free version of ab177466 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)
    Western blot - Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)
    All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : COIL knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab177466).

    Lanes 1-3: Merged signal (red and green). Green - ab177466 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.

    ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)
    Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)

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