Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)
![Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)](https://www.abcam.com/ps/products/249/ab249972/Images/ab249972-384528-anti-coilin-antibody-epr7843n-bsa-and-azide-free-western-blot-hela-jurkat.jpg "Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free
See all Coilin primary antibodies -
Description
Rabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat and HeLa cell lysates.
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General notes
ab249972 is the carrier-free version of ab177466 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7843(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COIL knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab177466).
Lanes 1-3: Merged signal (red and green). Green - ab177466 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)](https://www.abcam.com/ps/products/249/ab249972/Images/ab249972-2-benefits-of-recombinant-antibodies.png)
Anti-Coilin antibody [EPR7843(N)] - BSA and Azide free (ab249972)
