Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA6] to MAP2 - BSA and Azide free
  • Suitable for: IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAP2 antibody [AA6] - BSA and Azide free
    See all MAP2 primary antibodies

  • Description

    Mouse monoclonal [AA6] to MAP2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICCmore details
    Unsuitable for: Flow Cyt,IHC-Fr or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human astrocytoma. Mouse and rat cerebrum tissue. ICC: Rat and mouse primary neurons.

  • General notes

    ab255896 is the carrier-free version of ab254144. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255896 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

    This data was developed using ab254144 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human astrocytoma. The section was incubated with ab254144 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Immunocytochemistry - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

    This data was developed using ab254144 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

    Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

    This data was developed using ab254144 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

    This data was developed using ab254144 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Immunocytochemistry - Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

    This data was developed using ab254144 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

    Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
    Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)

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