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Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)

Overview

  • Product name

    Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human

  • Species reactivity

    Reacts with: Human

  • Product overview

    Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human ab254023 contains multiple trial-sized versions of anti-human antibody clones against CD31, CD34, ICAM1, CD45, specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies against human CD31, CD34, ICAM1, CD45 and 1 mouse monoclonal against human CD31. They are provided as a sampler panel to allow you to easily evaluate each in your required applications. 


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [EP3095] to CD31 (20 µL) ab134168


    - Mouse monoclonal [P2B1] to CD31 (20 µg) ab24590


    - Rabbit monoclonal [EP373Y] to CD34 (20 µL) ab81289


    - Rabbit monoclonal [EP1442Y] to ICAM1 (20 µL) ab53013


    - Rabbit monoclonal [EP322Y] to CD45 (20 µL) ab40763

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EP322Y]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EP322Y]

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab40763 at a working dilution of 1/250.

    The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (shown in the inset).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EP322Y]
    Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EP322Y]

    Immunofluorescence staining of Jurkat cells (Human T cell leukemia cell line from peripheral blood) with purified ab40763 at a working dilution of 1/100, counterstained with DAPI.

    The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y]

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ICAM1 with ab53013 at 1/1000. Heat mediated antigen retrieval was performed using ab93684, Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y]

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified ab81289 at 1/100-1/250.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [P2B1]
    Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [P2B1]

    Positive immunocytochemical staining of human primary choroidal endothelial cells using ab24590 at a dilution of 1/100. The tissue was fixed with paraformaldehyde and the antibody was incubated for 3 hours. An Alexa Fluor® 488 goat anti-mouse antibody was used as a secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095]
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095]

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma vessels tissue using ab134168 showing +ve staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y]
    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y]

    Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)
    Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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