Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (ab238602)
Overview
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Product name
Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2)
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Product overview
ab238602 is a Human Embyonic Stem Cell Marker Panel containing 50µg of Oct4 rabbit polyclonal, 50µg of Tra-1-60 mouse monoclonal, 50µl of Nanog rabbit polyclonal antibody and 50µg of SOX2 rabbit polyclonal antibody.
The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units ab21624 - Anti-Nanog antibody - ChIP Grade 1 x 50µl ab19857 - Anti-Oct4 antibody - ChIP Grade 1 x 50µg ab97959 - Anti-SOX2 antibody 1 x 50µg ab16288 - Anti-TRA-1-60 (R) antibody [TRA-1-60] 1 x 50µg -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab238602) This image is courtesy of an anonymous collaborator.
This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
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Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab238602) This image is courtesy of an anonymous collaborator.ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C.
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Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab238602) This image is courtesy of an anonymous collaborator.ICC/IF image of Nanog (ab21624) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.1% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21624, 1/300 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/500 dilution.
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Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (ab238602)
