Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60, SOX2, SSEA4) (ab109884)
Overview
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Product name
Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60, SOX2, SSEA4)
See all Human Embryonic Stem Cell Marker kits -
Product overview
Embryonic Stem Cell Marker Panel (Human) ab109884 contains 50µg of Oct4 rabbit polyclonal, 50µg of Tra-1-60 mouse monoclonal, 50µl of Nanog rabbit polyclonal antibody, 50µg of SOX2 rabbit polyclonal antibody and 50µl of SSEA4 mouse monoclonal antibodies.
The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains five antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2, SSEA4 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
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Notes
Panel contains:
- Oct4 rabbit polyclonal ab19857- 50µg
- Tra-1-60 mouse monoclonal ab16288- 50µg
- Nanog rabbit polyclonal antibody ab21624 - 50µl
- SOX2 rabbit polyclonal antibody ab97959 - 50µg
- SSEA4 mouse monoclonal ab16287- 50µl
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units ab21624 - Anti-Nanog antibody - ChIP Grade 1 x 50µl ab19857 - Anti-Oct4 antibody - ChIP Grade 1 x 50µg ab97959 - Anti-SOX2 antibody 1 x 50µg ab16287 - Anti-SSEA4 antibody [MC813] 1 x 50µl ab16288 - Anti-TRA-1-60 (R) antibody [TRA-1-60] 1 x 50µg -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884) This image is courtesy of an anonymous collaborator.This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
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Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884) This image is courtesy of an anonymous collaborator.ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C. -
Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884) This image is courtesy of an anonymous collaborator.NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst. -
Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884) This image is courtesy of an anonymous collaborator.ICC/IF image of Nanog (ab21624) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.1% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21624, 1/300 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/500 dilution. -
Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60, SOX2, SSEA4) (ab109884)
