Anti-WISP2 antibody (ab38317)
Key features and details
- Rabbit polyclonal to WISP2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WISP2 antibody
See all WISP2 primary antibodies -
Description
Rabbit polyclonal to WISP2 -
Host species
Rabbit -
Specificity
This antibody is specific for WISP2. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 127~141 of human WISP2.
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WISP2 antibody (ab38317)Anti-WISP2 antibody (ab38317) at 1/1000 dilution + A549 whole cell lysate at 35 µg/ml
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-WISP2 antibody (ab38317)ICC/IF image of ab38317 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-WISP2 antibody (ab38317)Anti-WISP2 antibody (ab38317) at 1/100 dilution +Recombinant human WISP2 protein (ab50040) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Exposure time: 10 seconds
