GANT 61, GLI1 and 2 inhibitor (ab120904)
Key features and details
- Selective GLI1 and 2 inhibitor
- CAS Number: 500579-04-4
- Purity: > 98%
- Soluble in DMSO to 50 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
GANT 61, GLI1 and 2 inhibitor -
Description
Selective GLI1 and 2 inhibitor -
Alternative names
- NSC 136476
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Biological description
Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo. -
Purity
> 98% -
CAS Number
500579-04-4 -
Chemical structure
Images
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Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.