Anti-SSEA3 antibody [MC631] (ab16286)
![Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.com/ps/products/16/ab16286/Images/ab16286-316783-anti-ssea3-antibody-mc631-flow-cytometry.jpg "Anti-SSEA3 antibody [MC631] (ab16286)")
Key features and details
- Rat monoclonal [MC631] to SSEA3
- Suitable for: IHC-FoFr, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-SSEA3 antibody [MC631] -
Description
Rat monoclonal [MC631] to SSEA3 -
Host species
Rat -
Tested applications
Suitable for: IHC-FoFr, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse SSEA3.
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Positive control
- 2102Ep Human Embryonic Carcinoma Cells
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated. -
Clonality
Monoclonal -
Clone number
MC631 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgM -
Research areas
Images
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Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286) This image is courtesy of Prof Chris Denning's lab, University of Nottingham
Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.com/ps/products/16/ab16286/Images/ab16286-24923-anti-ssea3-antibody-mc631-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286) This image is courtesy of the laboratory of Peter Andrews.The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286. -
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.com/ps/products/16/ab16286/Images/ab16286-24924-anti-ssea3-antibody-mc631-flow-cytometry.jpg)
Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286) This image is from an anonymous collaborator.Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).