Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-MAP2 (phospho S136) antibody [EPR2361]
See all MAP2 primary antibodies
Description

Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MAP2 (phospho S136).
Positive control
- WB: Human fetal brain and mouse brain tissue lysates IHC-P: Human brain tissue ICC/IF: Mouse primary neuron + Alkaline Phosphatase cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2361
Isotype

IgG
Research areas

Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution

Lane 1 : Untreated fetal brain lysates
Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase
Lane 3 : Mouse brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP/AP polymerized antibody

Predicted band size: 200 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com