Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP2 antibody [AA5] (ab254143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA5] to MAP2
  • Suitable for: IHC-P, IHC-Fr, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAP2 antibody [AA5]
    See all MAP2 primary antibodies

  • Description

    Mouse monoclonal [AA5] to MAP2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    Rat
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat cerebrum tissue. IHC-Fr: Mouse cerebrum tissue. ICC: Mouse and rat primary neurons.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254143 at 1/4000 dilution (0.289 µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254143 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [AA5] (ab254143)
    Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [AA5] (ab254143)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling MAP2 with ab254143 at 1/20 dilution (57.85 µg/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-MAP2 antibody [AA5] (ab254143)
    Immunocytochemistry - Anti-MAP2 antibody [AA5] (ab254143)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with ab254143 at 1/50 dilution (23.14 µg/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Negative control 1: ab254143 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

    Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254143 at 1/4000 dilution (0.289 µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254143 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA5] (ab254143)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254143 at 1/4000 dilution (0.289 µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254143 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-MAP2 antibody [AA5] (ab254143)
    Immunocytochemistry - Anti-MAP2 antibody [AA5] (ab254143)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with ab254143 at 1/50 dilution (23.14 µg/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Negative control 1: ab254143 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

    Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Anti-MAP2 antibody [AA5] (ab254143)
    Anti-MAP2 antibody [AA5] (ab254143)

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