Anti-NSE antibody (ab231114)
Key features and details
- Rabbit polyclonal to NSE
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NSE antibody
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken
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Immunogen
Recombinant full length protein corresponding to Mouse NSE aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P17183 -
Positive control
- IHC-P: Mouse brain tissue. WB: HepG2 and HEK-293T cell lysates; Recombinant mouse NSE protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231114 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (ab231114)
Formalin-fixed, paraffin-embedded mouse brain tissue stained for NSE using ab231114 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-NSE antibody (ab231114)Anti-NSE antibody (ab231114) at 2 µg/ml + Recombinant mouse NSE protein
Predicted band size: 47 kDa
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Western blot - Anti-NSE antibody (ab231114)All lanes : Anti-NSE antibody (ab231114) at 2 µg/ml
Lane 1 : Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : NSE knockout HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 47 kDa
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Western blot - Anti-NSE antibody (ab231114)Anti-NSE antibody (ab231114) at 2 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 47 kDa
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Western blot - Anti-NSE antibody (ab231114)Anti-NSE antibody (ab231114) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 47 kDa
