Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NSE antibody [EPR3377] - BSA and Azide free
    See all NSE primary antibodies

  • Description

    Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain tissue lysate and SH-SY-5Y, HeLa and Y79 cell lysates. IHC-P: Human brain tissue. ICC/IF: formaldehyde fixed SKNSH cells.

  • General notes

    ab220216 is the carrier-free version of ab79757. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220216 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    ICC/IF image of ab79757 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79757 at 1/25 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 ab79757.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Flow Cytometry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Flow Cytometry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    Overlay histogram showing HeLa cells stained with ab79757 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79757, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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