Anti-NSE antibody (ab134015)
Key features and details
- Chicken polyclonal to NSE
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgY
Overview
-
Product name
Anti-NSE antibody
See all NSE primary antibodies -
Description
Chicken polyclonal to NSE -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
-
Immunogen
Two synthetic peptides, conjugated to KLH, corresponding to different regions of the NSE gene product, but shared between the Human (NP_001966, NCBI) and Rat (AAA41119, NCBI) sequences.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of each of the two affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Western blot - Anti-NSE antibody (ab134015)Anti-NSE antibody (ab134015) at 1/5000 dilution + Adult mouse brain solubilized with Lamelli cracking buffer
Predicted band size: 47 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (ab134015)Immunohistochemical analysis of dissociated cell cultures of neonatal mouse brains, labelling NSE-2 (green staining) in neurons using ab134015 at 1/2000 dilution. Cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.
