Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2361] to MAP2 (phospho S136) - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free
    See all MAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2361] to MAP2 (phospho S136) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse primary neuron + Alkaline Phosphatase cells

  • General notes

    Ab247601 is the carrier-free version of ab96378. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab96378).

  • Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)
    Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)

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