Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes
Suitable for: IHC-P, IHC-Fr, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-TBR2 / Eomes antibody [EPR21950-241]
See all TBR2 / Eomes primary antibodies
Description

Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, E14.5 mouse cerebral cortex and E14.5 rat cerebral cortex tissues. ICC/IF: NK-92 cells. IHC-Fr: Mouse E14.5 cerebrum and Rat E14.5 cerebrum tissue. IP: NK-92 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21950-241
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat E14.5 cerebrum tissue labeling EOMES with ab216870 at 1/100 (5.75 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining in rat embryonic cerebrum (PMID: 24223221) is observed.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunohistochemical analysis of paraffin-embedded E14.5 rat cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 rat cerebral cortex (PMID: 18725516) is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labelling EOMES with ab216870 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NK-92 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

TBR2 / Eomes was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate 10μg with ab216870 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab216870 1/500 dilution (1.2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

Lane 1: NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate 10μg

Lane 2: ab216870 IP in NK-92 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216870 in NK-92 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min

Lysates were made fresh and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

Under these experimental conditions ab216870 enriches for the 75kDa Eomes protein in IP.

This band size (75kDa) is consistent with what has been described in the literature (PMID: 26749212).
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum tissue stained for EOMES using ab216870 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Positive staining in mouse embryonic cerebrum (PMID: 24223221) is observed. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunohistochemical analysis of paraffin-embedded E14.5 mouse cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 mouse cerebral cortex (PMID: 18725516) The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

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