Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free
    See all TBR2 / Eomes primary antibodies

  • Description

    Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, E14.5 mouse cerebral cortex and E14.5 rat cerebral cortex tissues. IHC-Fr: Mouse E14.5 cerebrum and Rat E14.5 cerebrum tissue.

  • General notes

    ab261913 is the carrier-free version of ab216870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261913 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat E14.5 cerebrum tissue labeling EOMES with ab216870 at 1/100 (5.75 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining in rat embryonic cerebrum (PMID: 24223221) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunohistochemical analysis of paraffin-embedded E14.5 rat cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 rat cerebral cortex (PMID: 18725516) is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cells labelling EOMES with ab216870 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NK-92 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    TBR2 / Eomes was immunoprecipitated from 0.35 mg NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate 10μg with ab216870 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab216870 1/500 dilution (1.2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate 10μg

    Lane 2: ab216870 IP in NK-92 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216870 in NK-92 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    Lysates were made fresh and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

    Under these experimental conditions ab216870 enriches for the 75kDa Eomes protein in IP.

    This band size (75kDa) is consistent with what has been described in the literature (PMID: 26749212).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum tissue stained for EOMES using ab216870 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was  ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Positive staining in mouse embryonic cerebrum (PMID: 24223221) is observed. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunohistochemical analysis of paraffin-embedded E14.5 mouse cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 mouse cerebral cortex (PMID: 18725516) is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216870).

  • Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)
    Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free (ab261913)

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