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Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22665-104] to ErbB4 / HER4 - BSA and Azide free
  • Suitable for: ELISA, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free
    See all ErbB4 / HER4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22665-104] to ErbB4 / HER4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab263017 is not recommended for mouse IHC.

  • Tested applications

    Suitable for: ELISA, WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, T-47D, MCF7 and 4T1 lysates. IHC-P: Human breast carcinoma tissue. Flow Cyt: T-47D and MCF7 cells.

  • General notes

    ab263027 is the carrier-free version of ab219208. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab263027 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB 4 with ab219208 at 1/1000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the human breast carcinoma (PMID: 19239686) is observed. The section was incubated with ab219208 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219208).

  • Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)
    Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling ErbB 4 with ab219208 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219208).

  • Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)
    Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (Human ductal breast epithelial tumor epithelial cell) cells labelling ErbB 4 with ab219208 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219208).

  • ELISA - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)
    ELISA - Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

    This data was developed using ab219208, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human ERBB4 recombinant protein at 1000 ng/mL with ab219208. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)
    Anti-ErbB4 / HER4 antibody [EPR22665-104] - BSA and Azide free (ab263027)

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