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Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22665-104] to ErbB4 / HER4
  • Suitable for: ELISA, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-ErbB4 / HER4 antibody [EPR22665-104]
    See all ErbB4 / HER4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22665-104] to ErbB4 / HER4

  • Host species

    Rabbit

  • Specificity

    ab219208 is not recommended for mouse IHC.

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, T-47D, MCF7 and 4T1 lysates. IHC-P: Human breast carcinoma tissue. Flow Cyt: T-47D and MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB4 / HER4 with ab219208 at 1/1000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the human breast carcinoma (PMID: 19239686) is observed. The section was incubated with ab219208 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    Western blot - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    All lanes : Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 147 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22761786)  Lane 4 was developed using a higher sensitivity ECL substrate. 

    Exposure time: Lane 1:70 seconds; Lanes 2-3:48 seconds; Lane 4: 3 minutes.

  • Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling ErbB4 / HER4 with ab219208 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    Flow Cytometry - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (Human ductal breast epithelial tumor epithelial cell) cells labelling ErbB4 / HER4 with ab219208 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • ELISA - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    ELISA - Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    ELISA analysis of Human ERBB4 recombinant protein at 1000 ng/mL with ab219208. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)
    Anti-ErbB4 / HER4 antibody [EPR22665-104] (ab219208)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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