Anti-ErbB4 / HER4 (phospho Y1188) antibody [EPR2271Y] (ab68479)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2271Y] to ErbB4 / HER4 (phospho Y1188)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1188) antibody [EPR2271Y]
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit monoclonal [EPR2271Y] to ErbB4 / HER4 (phospho Y1188) -
Host species
Rabbit -
Specificity
This antibody detects ErbB 4 phosphorylated on tyrosine 1188. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
corresponding to Human ErbB4/ HER4. A phospho-peptide corresponding to residues surrounding tyrosine 1188 of human ErbB4/ HER4.
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Positive control
- A431 cell lysate, cells treated with EGF.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2271Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB4 / HER4 (phospho Y1188) antibody [EPR2271Y] (ab68479) at 1/2000 dilution
Lane 1 : A431 cell lysate. Cells untreated.
Lane 2 : A431 cell lysate. Cells treated with EGF.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 170-200 kDa why is the actual band size different from the predicted?
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