Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162) - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free
    See all ErbB4 / HER4 primary antibodies

  • Description

    Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects ErbB 4 phosphorylated on tyrosine 1162. 

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247386 is the carrier-free version of ab68478 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247386 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)
    Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)
    All lanes : Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478) at 1/500 dilution

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 147 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab68478, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)
    This data was developed using ab68478, the same antibody clone in a different buffer formulation.Human breast carcinoma stained with ab68478 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)
    Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)

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