Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)
![Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)](https://www.abcam.com/ps/products/275/ab275386/Images/ab275386-1-anti-hhla2-antibody-epr23563120-flow-cytometry-hela.jpg "Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23563-120] to HHLA2 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free
See all HHLA2 primary antibodies -
Description
Rabbit monoclonal [EPR23563-120] to HHLA2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: A-204 cells; HEK-293T cells transfected with Myc-His tagged HHLA2 overexpression vector.
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General notes
ab275386 is the carrier-free version of ab254367. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275386 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23563-120 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)
This data was developed using ab254367, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell line, left) / A-204 (human muscle rhabdomyosarcoma, right) cells labelling HHLA2 with ab254367 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Hela (PMID:23716685).
Gated on viable cells.
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![Flow Cytometry - Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)](https://www.abcam.com/ps/products/275/ab275386/Images/ab275386-2-anti-hhla2-antibody-epr23563120-flow-cytometry-293t.jpg)
Flow Cytometry - Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)This data was developed using ab254367, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell line) cells transfected with Myc-His tagged HHLA2 overexpression vector labelling HHLA2 with ab254367 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG or ab254367. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor®647.
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![Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)](https://www.abcam.com/ps/products/275/ab275386/Images/ab275386-3-benefits-of-recombinant-antibodies.png)
Anti-HHLA2 antibody [EPR23563-120] - BSA and Azide free (ab275386)
