Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP84] to EGFR - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR antibody [SP84] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [SP84] to EGFR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung squamous cell carcinoma, breast ductal carcinoma, colon adenocarcinoma, lung adenocarcinoma, renal cell carcinoma, stomach adenocarcinoma, liver hepatocellular carcinoma, bladder transitional cell carcinoma, placenta and liver tissues. Flow Cyt: A431 cells. ICC/IF: A431 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab238803 is the carrier-free version of ab227642. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238803 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Flow Cytometry - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Flow Cytometry - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Flow Cytometry analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:200 dilution (0.48 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227635).
  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunocytochemistry/ Immunofluorescence analysis of A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:50 (1.92 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human piver tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227642).

  • Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)
    Anti-EGFR antibody [SP84] - BSA and Azide free (ab238803)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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