Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992) - BSA and Azide free
  • Suitable for: ICC/IF, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab229195 is the carrier-free version of ab151704 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab229195 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)
    Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

    Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151704).

  • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)
    Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)

    Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151704).

  • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)
    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)

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