Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

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Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20175] to hnRNP A1 (citrulline R88 + R92)
Suitable for: Flow Cyt, IP, WB, Dot blot
Reacts with: Human

Product name

Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175]
See all hnRNP A1 primary antibodies
Description

Rabbit monoclonal [EPR20175] to hnRNP A1 (citrulline R88 + R92)
Host species

Rabbit
Specificity

This antibody cross reacts with hnRNP A1L2 (citrulline R88/92), hnRNP A0 (citrulline R81/85) and hnRNP A2B1 (citrulline R95/99).

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with PADI4 (WT) and HEK-293T transfected with PADI2 (WT) whole cell lysates treated with CaCl2 and Ionomycin. Flow Cyt: HEK-293T transfected with PADI4 (WT) cells treated with CaCl2 and Ionomycin. IP: HEK-293T transfected with PADI4 (WT) cell lysate treated with CaCl2 and Ionomycin.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20175
Isotype

IgG
Research areas

Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

All lanes : Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027) at 1/5000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T transfected with a PADI4 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 3 : HEK-293T transfected with an empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : HEK-293Ttransfected with a PADI2 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 31,37,39 kDa
why is the actual band size different from the predicted?

hnRNP A1(Uniprot P09651), isoform A1-A (34kD) is twenty times more abundant than isoform A1-B (39kD). The molecular weights of hnRNP A1L2 (Q32P51), hnRNP A0 (Q13151) and hnRNP A2B1 (P22626) are 34kD, 31kD and 37kD respectively.

PAD enzymes (including PADI2 and PADI4) hydrolyze arginine to form citrulline, Ca²⁺ and ionomycin are also required for this catalytic reaction (PMID: 26360112).

Blocking/Dilution buffer: 5% NFDM/TBST

Exposure times: Lane 1-2: 1 second; Lane 3-4: 2 seconds.
Dot Blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

Dot Blot analysis of hnRNP A1 (citrulline R88 + R92) labeled with ab208027 at 1/1000 dilution.

Lane 1: hnRNP A1 (citrulline R88/92) peptide.

Lane 2: hnRNP A3 (citrulline R109/113) peptide.

Lane 3: hnRNP A0 (citrulline R81/85) peptide.

Lane 4: hnRNP A2B1 (citrulline R95/99) peptide.

Lane 5: CCDC51 (citrulline R142) peptide.

Lane 6: hnRNP A1 non-citrulline peptide.

Lane 7: hnRNP A1 (citrulline R88) peptide.

Lane 8: hnRNP A1 (citrulline R92) peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.
Flow Cytometry - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10 μM Ionomycin for 2 hours, labeling hnRNP A1 with ab208027 at 1/5000 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

The CaCl₂ and Ionomycin treated GFP positive cell population gives a positive signal. (Right panel, Q2).
Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

hnRNP A1 was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) with ab208027 at 1/30 dilution, whole cell lysate. Western blot was performed from the immunoprecipitate using ab208027 at 1/10000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10μM Ionomycin for 2 hours whole cell lysate 10 μg (Input).

Lane 2: ab208027 IP in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10 μM Ionomycin for 2 hours whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208027 in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl₂ and 10 μM Ionomycin for 2 hours whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027)

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