Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92)
Suitable for: WB, Dot blot, Flow Cyt, IP
Reacts with: Human

Product name

Anti-hnRNP A1 (citrulline R92) antibody [EPR20174]
See all hnRNP A1 primary antibodies
Description

Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with GFP-tagged PADI2 and PADI4, respectively, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysates. Flow Cyt: HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, cells. IP: HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20174
Isotype

IgG
Research areas

Western blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

All lanes : Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026) at 1/2000 dilution

Lanes 1 & 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

According to Uniprot annotation for HNRNPA1, isoform A1-A (34 kDa) is twenty times more abundant than isoform A1-B (39 kDa). The MW of HNRNPA1L2 is also 34 kD.

In WB this antibody does not cross react with CCDC51 (expected MW 45 kDa).
Dot Blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

Dot blot analysis of hnRNP A1 (citrulline R92) labeled with ab208026 at 1/1000 dilution.

Lane 1: hnRNP A1 (citrulline R92) peptide.

Lane 2: hnRNP A1 non-citrulline peptide.

Lane 3: hnRNP A3 (citrulline R113) peptide.

Lane 4: hnRNP A0 (citrulline R85) peptide.

Lane 5: CCDC51(citrulline R142) peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Based on sequence homology this antibody cross reacts with CCDC51 (citrulline R142) peptide (detected by dot blot only) and hnRNP A1L2 (citrulline R92) protein.

Exposure time: 3 minutes.

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, labeling hnRNP A1 (citrulline R92) with ab208026 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) (150079) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

hnRNP A1 (citrulline R92) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate with ab208026 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate 10 µg (Input).

Lane 2: ab208026 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208026 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.

Exposure time: Less than 1 second.

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)

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