Anti-hnRNP A1 antibody (ab137780)
Key features and details
- Rabbit polyclonal to hnRNP A1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP A1 antibody
See all hnRNP A1 primary antibodies -
Description
Rabbit polyclonal to hnRNP A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Monkey -
Immunogen
Recombinant fragment, corresponding to amino acids 1-214 of Human hnRNP A1 (UniProt ID: P09651).
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Positive control
- Mouse brain and 293T, A431, H1299, HeLaS3, Hela, HepG2, Molt4 and Raji cell lysates; HeLa cells; Ca922 xenograft
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/3000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : hnRNP A1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa
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All lanes : Anti-hnRNP A1 antibody (ab137780) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HelaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
12% SDS PAGE -
Anti-hnRNP A1 antibody (ab137780) at 1/1000 dilution + Mouse brain cell lysate at 50 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling hnRNP A1 with ab137780 at a 1/200 dilution. The lower image (labeled '2') was merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded Ca922 xenograft, labelling hnRNP A1 using ab137780 at a 1/100 dilution.