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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PAK4 antibody (ab62509)

Price and availability

301 536 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-PAK4 antibody (ab62509)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PAK4
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PAK4 antibody
    See all PAK4 primary antibodies
  • Description

    Rabbit polyclonal to PAK4
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with PAK4
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A 13 amino acid synthetic peptide from near the center of human PAK4

  • Positive control

    • SW480 cell lysate and human colon tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Western blot - Anti-PAK4 antibody (ab62509)
    Western blot - Anti-PAK4 antibody (ab62509)
    Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
    Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml

    All lanes : SW480 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody (ab62509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody (ab62509)
    Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)
    Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)
    ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)
    Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)
    Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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