Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7816] to MED4 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MED4 antibody [EPR7816] - BSA and Azide free
    See all MED4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7816] to MED4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248327 is the carrier-free version of ab129170. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    Western blot - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    All lanes : Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : T-47D cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 30 kDa



    This data was developed using ab129170, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    This data was developed using ab129170, the same antibody clone in a different buffer formulation.ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    Immunocytochemistry - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    This data was developed using ab129170, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    This data was developed using ab129170, the same antibody clone in a different buffer formulation.ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    OI-RD Scanning - Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    This data was developed using ab129170, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)
    Anti-MED4 antibody [EPR7816] - BSA and Azide free (ab248327)

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