Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
![Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-5-anti-erh-antibody-epr10830-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10830(B)] to ERH - BSA and Azide free
- Suitable for: ICC, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ERH antibody [EPR10830(B)] - BSA and Azide free
See all ERH primary antibodies -
Description
Rabbit monoclonal [EPR10830(B)] to ERH - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249359 is the carrier-free version of ab166620. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249359 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10830(B) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)This data was developed using ab166620, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling ERH with ab166620 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-408101-anti-erh-antibody-epr10830-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)All lanes : Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution
Lane 1 : T-47D cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : A549 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDaThis data was developed using ab166620, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-3-anti-erh-antibody-epr10830-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)This data was developed using ab166620, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ERH with ab166620 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-2-anti-erh-antibody-epr10830-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359) -
![Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-399691-anti-erh-antibody-epr10830-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)This data was developed using ab166620, the same antibody clone in a different buffer formulation.
Immunoprecipitation analysis of ERH in pellet from A549 cell lysate using ab166620 at 1/10 dilution, followed by WB detection.
Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution + immunoprecipitation pellet from A549 cell lysate at 10 µg
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![Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)](https://www.abcam.com/ps/products/249/ab249359/Images/ab249359-6-benefits-of-recombinant-antibodies.png)
Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)