Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12768] to hnRNP A1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-hnRNP A1 antibody [EPR12768]
See all hnRNP A1 primary antibodies
Description

Rabbit monoclonal [EPR12768] to hnRNP A1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP A1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P09651
Positive control
- MCF-7, HepG2, K562, HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). Human breast carcinoma and clear cell carcinoma tissue. HepG2 cells. Permeabilized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12768
Isotype

IgG
Research areas

Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : hnRNP A1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 38 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Flow cytometric analysis of permeabilized Jurkat cells labeling hnRNP A1 with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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