HeLa whole cell lysate (ab29545)
Overview
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Product name
HeLa whole cell lysate
See all HeLa lysates -
General notes
Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Allow to warm to room temp and agitate gently before aliquotting. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1% 4-Nonylphenol, branched, ethoxylated, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% Sodium lauryl sulfate, HEPES, Sucrose, 10% Glycerol
With Protease inhibitor cocktail/EDTA/Sodium deoxycholate -
Concentration information loading... -
Lysate notes
Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
Research areas
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Background
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
