Anti-EpCAM antibody [4A7] - C-terminal (ab224826)
![Anti-EpCAM antibody [4A7] - C-terminal (ab224826)](https://www.abcam.com/ps/products/224/ab224826/Images/ab224826-285989-anti-epcam-antibody-4a7-c-terminal-immunohistochemistry.jpg "Anti-EpCAM antibody [4A7] - C-terminal (ab224826)")
Key features and details
- Mouse monoclonal [4A7] to EpCAM - C-terminal
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-EpCAM antibody [4A7] - C-terminal
See all EpCAM primary antibodies -
Description
Mouse monoclonal [4A7] to EpCAM - C-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human EpCAM. (From intracellular domain of the C-terminus).
Database link: P16422 -
Positive control
- WB: Wild-type HAP1, MCF7, and HT-29 cell lysates. IHC-P: Human colon adenocarcinoma tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
4A7 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [4A7] - C-terminal (ab224826)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EpCAM using ab224826 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-EpCAM antibody [4A7] - C-terminal (ab224826)](https://www.abcam.com/ps/products/224/ab224826/Images/ab224826-369831-anti-epcam-antibody-4a7-c-terminal-western-blot-a431-wt-a431-epcam-knockout-mcf7-hela.jpg)
Western blot - Anti-EpCAM antibody [4A7] - C-terminal (ab224826)All lanes : Anti-EpCAM antibody [4A7] - C-terminal (ab224826) at 1/400 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : EPCAM knockout A431 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDaLanes 1 - 4: Merged signal (red and green). Green - ab224826 observed at 35 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab224826 was shown to react with EpCAM in A431 wild-type cells in Western blot. Loss of signal was observed when EpCAM knockout sample was used. A431 wild-type and EpCAM knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab224826 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-EpCAM antibody [4A7] - C-terminal (ab224826)](https://www.abcam.com/ps/products/224/ab224826/Images/ab224826-285987-anti-epcam-antibody-4a7-c-terminal-western-blot.jpg)
Western blot - Anti-EpCAM antibody [4A7] - C-terminal (ab224826)Anti-EpCAM antibody [4A7] - C-terminal (ab224826) at 1/100 dilution + HT-29 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 35 kDa
