Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)
![Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)](https://www.abcam.com/ps/products/271/ab271981/Images/ab271981-2-ab208028289358antihnrnpa1citrulliner97antibodyepr20176dotblot.jpg "Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free
- Suitable for: IP, WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on sequence homology we predict that this antibody may react with hnRNP A1L2.
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Tested Applications & Species
See all applications and species dataApplication Species Dot Recombinant fragmentIP Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with PADI2 and PADI4, respectively, treated with CaCl2 and Ionomycin, whole cell lysates. IP: HEK-293T transfected with PADI4, treated with CaCl2 and Ionomycin, whole cell lysate.
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General notes
ab271981 is the carrier-free version of ab208028. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20176 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
Dot blot analysis of hnRNP A1 (citrulline R97) labeled with ab208028 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R97) peptide.
Lane 2:hnRNP A1 (citrulline R97) non-citrulline peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028). -
![Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)](https://www.abcam.com/ps/products/271/ab271981/Images/ab271981-1-ab208028289356antihnrnpa1citrulliner97antibodyepr20176immunoprecipitation.jpg)
Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)hnRNP A1 (citrulline R97) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate with ab208028 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208028 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input).
Lane 2: ab208028 IP in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208028 in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.Exposure time: 10 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028).
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![Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)](https://www.abcam.com/ps/products/271/ab271981/Images/ab271981-3-benefits-of-recombinant-antibodies.png)
Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)
