Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20175] to hnRNP A1 (citrulline R88 + R92) - BSA and Azide free
  • Suitable for: Dot blot, Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free
    See all hnRNP A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20175] to hnRNP A1 (citrulline R88 + R92) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody cross reacts with hnRNP A1L2 (citrulline R88/92), hnRNP A0 (citrulline R81/85) and hnRNP A2B1 (citrulline R95/99).

  • Tested applications

    Suitable for: Dot blot, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251486 is the carrier-free version of ab208027. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20175

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    Western blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    All lanes : Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] (ab208027) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 2 : HEK-293T transfected with a PADI4 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 3 : HEK-293T transfected with an empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 4 : HEK-293T transfected with a PADI2 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 31,37,39 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab208027, the same antibody clone in a different buffer formulation.

    hnRNP A1 (Uniprot P09651), isoform A1-A (34kD) is twenty times more abundant than isoform A1-B (39kD). The molecular weights of hnRNP A1L2 (Q32P51), hnRNP A0 (Q13151) and hnRNP A2B1 (P22626) are 34kD, 31kD and 37kD respectively.

    PAD enzymes (including PADI2 and PADI4) hydrolyze arginine to form citrulline, Ca2+ and ionomycin are also required for this catalytic reaction (PMID: 26360112).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 second; Lanes 3-4: 2 seconds.

  • Dot Blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    Dot Blot - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    This data was developed using ab208027, the same antibody clone in a different buffer formulation.Dot Blot analysis of hnRNP A1 (citrulline R88 + R92) labeled with ab208027 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R88/92) peptide. Lane 2: hnRNP A3 (citrulline R109/113) peptide. Lane 3: hnRNP A0 (citrulline R81/85) peptide. Lane 4: hnRNP A2B1 (citrulline R95/99) peptide. Lane 5: CCDC51 (citrulline R142) peptide. Lane 6: hnRNP A1 non-citrulline peptide. Lane 7: hnRNP A1 (citrulline R88) peptide. Lane 8: hnRNP A1 (citrulline R92) peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Flow Cytometry - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    Flow Cytometry - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    This data was developed using ab208027, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours, labeling hnRNP A1 with ab208027 at 1/5000 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody. The CaCl2 and Ionomycin treated GFP positive cell population gives a positive signal. (Right panel, Q2).
  • Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    Immunoprecipitation - Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)

    This data was developed using ab208027, the same antibody clone in a different buffer formulation.

    hnRNP A1 was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) with ab208027 at 1/30 dilution, whole cell lysate. Western blot was performed from the immunoprecipitate using ab208027 at 1/10000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10µM Ionomycin for 2 hours whole cell lysate 10 µg (Input).

    Lane 2: ab208027 IP in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208027 in HEK-293T transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)
    Anti-hnRNP A1 (citrulline R88 + R92) antibody [EPR20175] - BSA and Azide free (ab251486)

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