Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92) - BSA and Azide free
  • Suitable for: Dot blot, Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free
    See all hnRNP A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251485 is the carrier-free version of ab208026. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251485 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR20174

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    Western blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    All lanes : Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026) at 1/2000 dilution

    Lanes 1 & 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 5 seconds


    This data was developed using ab208026, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. According to Uniprot annotation for HNRNPA1, isoform A1-A (34 kDa) is twenty times more abundant than isoform A1-B (39 kDa). The MW of HNRNPA1L2 is also 34 kD. In WB this antibody does not cross react with CCDC51 (expected MW 45 kDa).

  • Dot Blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    Dot Blot - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    This data was developed using ab208026, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R92) labeled with ab208026 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R92) peptide. Lane 2: hnRNP A1 non-citrulline peptide. Lane 3: hnRNP A3 (citrulline R113) peptide. Lane 4: hnRNP A0 (citrulline R85) peptide. Lane 5: CCDC51(citrulline R142) peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Based on sequence homology this antibody cross reacts with CCDC51 (citrulline R142) peptide (detected by dot blot only) and hnRNP A1L2 (citrulline R92) protein. Exposure time: 3 minutes. Blocking and dilution buffer: 5% NFDM/TBST.
  • Flow Cytometry - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    Flow Cytometry - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    This data was developed using ab208026, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, labeling hnRNP A1 (citrulline R92) with ab208026 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (150079) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    Immunoprecipitation - Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    This data was developed using ab208026, the same antibody clone in a different buffer formulation.hnRNP A1 (citrulline R92) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate with ab208026 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate 10 µg (Input). Lane 2: ab208026 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208026 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate. Exposure time: Less than 1 second. Blocking and dilution buffer: 5% NFDM/TBST.
  • Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)
    Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] - BSA and Azide free (ab251485)

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