Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)
![Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)](https://www.abcam.com/ps/products/255/ab255280/Images/ab255280-4-anti-cd229-antibody-epr22611-91-immunohistochemistry-spleen-rat.jpg "Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22611-91] to CD229 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD229 antibody [EPR22611-91] - BSA and Azide free
See all CD229 primary antibodies -
Description
Rabbit monoclonal [EPR22611-91] to CD229 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human lymph node lysate; mouse lymph node lysate; rat thymus and lymph node lysates; A20, EL4, WEHI-231, Daudi and Jurkat whole cell lysates. IHC-P: Human colon and multiple myeloma tissue; mouse spleen tissue; rat spleen tissue.
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General notes
Ab255280 is the carrier-free version of ab252931. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255280 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22611-91 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of rat spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252931).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)](https://www.abcam.com/ps/products/255/ab255280/Images/ab255280-3-anti-cd229-antibody-epr22611-91-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of mouse spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252931).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)](https://www.abcam.com/ps/products/255/ab255280/Images/ab255280-2-anti-cd229-antibody-epr22611-91-immunohistochemistry-multiple-myeloma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining in tumor cells of human multiple myeloma (PMID: 21606160) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252931).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)](https://www.abcam.com/ps/products/255/ab255280/Images/ab255280-1-anti-cd229-antibody-epr22611-91-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of human colon (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252931).
-
![Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)](https://www.abcam.com/ps/products/255/ab255280/Images/ab255280-5-benefits-of-recombinant-antibodies.png)
Anti-CD229 antibody [EPR22611-91] - BSA and Azide free (ab255280)
