Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD229 antibody [EPR22611-54] (ab254266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22611-54] to CD229
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD229 antibody [EPR22611-54]
    See all CD229 primary antibodies

  • Description

    Rabbit monoclonal [EPR22611-54] to CD229

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CD229 aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9HBG7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow cyt: Daudi cells and human PBMCs.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-CD229 antibody [EPR22611-54] (ab254266)
    Flow Cytometry - Anti-CD229 antibody [EPR22611-54] (ab254266)

    Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) (Left) and Daudi (human Burkitt's lymphoma cell line) (Right) cells labeling CD229 with ab254266 at 1/500 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Negative control: U937. The expression pattern is consistent with what has been described in the literature. (PMID: 15245368).

    Gated on viable cells.

  • Flow Cytometry - Anti-CD229 antibody [EPR22611-54] (ab254266)
    Flow Cytometry - Anti-CD229 antibody [EPR22611-54] (ab254266)

    Flow cytometric analysis of human PBMC (peripheral blood mononuclear cells) cells labeling CD229 with ab254266 at 1/500 (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). 

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab254266 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

    The expression pattern is consistent with what described in the literature. (PMID: 11369645)

    Gated on viable cells.

  • Anti-CD229 antibody [EPR22611-54] (ab254266)
    Anti-CD229 antibody [EPR22611-54] (ab254266)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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