Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD229 antibody [EPR22611-91] (ab252931)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22611-91] to CD229
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD229 antibody [EPR22611-91]
    See all CD229 primary antibodies

  • Description

    Rabbit monoclonal [EPR22611-91] to CD229

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lymph node lysate; mouse lymph node lysate; rat thymus and lymph node lysates; A20, EL4, WEHI-231, Daudi and Jurkat whole cell lysates. IHC-P: Human colon and multiple myeloma tissue; mouse spleen tissue; rat spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)
    All lanes : Anti-CD229 antibody [EPR22611-91] (ab252931) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : U937 (human histiocytic lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 72 kDa
    Observed band size: 70-120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11369645; 17919264)).

    The bands between 70-120 kDa are caused by isoforms and glycosylation.

    Negative control: U-937 (PMID:15245368).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of rat spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)
    All lanes : Anti-CD229 antibody [EPR22611-91] (ab252931) at 1/1000 dilution

    Lane 1 : A20 (mouse reticulum sarcoma cell line) whole cell lysate
    Lane 2 : EL4 (mouse lymphoma T lymphocyte cell line) whole cell lysate
    Lane 3 : WEHI-231 (mouse lymphoblast B cell lymphoma cell line) whole cell lysate
    Lane 4 : Rat thymus lysate
    Lane 5 : Mouse lymph node lysate
    Lane 6 : Rat lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 72 kDa
    Observed band size: 70-120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 6 seconds; lane 2: 8 seconds; Lane 3: 48 seconds; Lanes 4 & 5: 15 seconds.; Lane 6 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11369645; 17919264).

     The bands between 70-120 kDa are due to isoforms and glycosylation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of mouse spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

    Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining in tumor cells of human multiple myeloma (PMID: 21606160) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD229 with ab252931 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of human colon (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-CD229 antibody [EPR22611-91] (ab252931)
    Anti-CD229 antibody [EPR22611-91] (ab252931)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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