Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD229 antibody [EPR22611-91] (ab252931)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22611-91] to CD229
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-CD229 antibody [EPR22611-91]
See all CD229 primary antibodies
Description

Rabbit monoclonal [EPR22611-91] to CD229
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human lymph node lysate; mouse lymph node lysate; rat thymus and lymph node lysates; A20, EL4, WEHI-231, Daudi and Jurkat whole cell lysates. IHC-P: Human colon and multiple myeloma tissue; mouse spleen tissue; rat spleen tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22611-91
Isotype

IgG
Research areas

Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)

All lanes : Anti-CD229 antibody [EPR22611-91] (ab252931) at 1/1000 dilution

Lane 1 : Human lymph node lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : U937 (human histiocytic lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 72 kDa
Observed band size: 70-120 kDa
why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 11369645; 17919264)).

The bands between 70-120 kDa are caused by isoforms and glycosylation.

Negative control: U-937 (PMID:15245368).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of rat spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-CD229 antibody [EPR22611-91] (ab252931)

All lanes : Anti-CD229 antibody [EPR22611-91] (ab252931) at 1/1000 dilution

Lane 1 : A20 (mouse reticulum sarcoma cell line) whole cell lysate
Lane 2 : EL4 (mouse lymphoma T lymphocyte cell line) whole cell lysate
Lane 3 : WEHI-231 (mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lane 4 : Rat thymus lysate
Lane 5 : Mouse lymph node lysate
Lane 6 : Rat lymph node lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 72 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 6 seconds; lane 2: 8 seconds; Lane 3: 48 seconds; Lanes 4 & 5: 15 seconds.; Lane 6 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID: 11369645; 17919264).

The bands between 70-120 kDa are due to isoforms and glycosylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of mouse spleen (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling CD229 with ab252931 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining in tumor cells of human multiple myeloma (PMID: 21606160) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD229 antibody [EPR22611-91] (ab252931)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD229 with ab252931 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in lymphocytes of human colon (PMID: 17919264; 11369645) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD229 antibody [EPR22611-91] (ab252931)

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