Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22611-54] to CD229 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD229 antibody [EPR22611-54] - BSA and Azide free
    See all CD229 primary antibodies

  • Description

    Rabbit monoclonal [EPR22611-54] to CD229 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Daudi cells and human PBMCs.

  • General notes

    Ab255689 is the carrier-free version of ab254266. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255689 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)
    Flow Cytometry - Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)

    Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) (Left) and Daudi (human Burkitt's lymphoma cell line) (Right) cells labeling CD229  with ab254266 at 1/500 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Negative control: U937. The expression pattern is consistent with what has been described in the literature. (PMID: 15245368).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254266).

  • Flow Cytometry - Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)
    Flow Cytometry - Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)

    Flow cytometric analysis of human PBMC (peripheral blood mononuclear cells) cells labeling CD229 with ab254266 at 1/500 (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). 

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab254266 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

    The expression pattern is consistent with what described in the literature. (PMID: 11369645)

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254266).

  • Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)
    Anti-CD229 antibody [EPR22611-54] - BSA and Azide free (ab255689)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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