Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12683] to Histone H1.3 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free
    See all Histone H1.3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12683] to Histone H1.3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • T47-D, Hela & Jurkat cell lysates, Human cervix carcinoma tissue, HeLa & T47-D cells

  • General notes

    Ab203948 is the carrier-free version of ab183736. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab203948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Western blot - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution

    Lane 1 : T-47D cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab183736, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Flow Cytometry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    This data was developed using ab183736, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
  • Immunocytochemistry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Immunocytochemistry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    This data was developed using ab183736, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
  • Immunocytochemistry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Immunocytochemistry - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    This data was developed using ab183736, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    This data was developed using ab183736, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
    Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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