Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 02, 2021

Anti-mH2A1 antibody [EPR9358] (ab137117)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9358] to mH2A1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-mH2A1 antibody [EPR9358]
See all mH2A1 primary antibodies
Description

Rabbit monoclonal [EPR9358] to mH2A1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human mH2A1 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- Fetal kidney, HepG2, HeLa, and MCF7 cell lysates, Human cervical carcinoma and Human colon tissue.
General notes
Previously labelled as macroH2A.1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9358
Isotype

IgG
Research areas

Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: mH2A1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hepg2 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab137117 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab137117 was shown to recognize mH2A1 when mH2A1 knockout samples were used, along with additional cross-reactive bands. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. Ab137117 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117) This image is courtesy of an anonymous abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver from wild-type and mH2A1/2 knock out tissue sections labeling mH2A1 with ab137117 at 1/400 dilution. Sections were fixed in formaldehyde; heat mediated antigen retivial was performed using a citrate buffer pH 6. An undiluted polyclonal horse anti-rabbit IgG (HRP-conjugated) was used as the secondary antibody.
See Abreview
Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117)

All lanes : Anti-mH2A1 antibody [EPR9358] (ab137117) at 1/1000 dilution

Lane 1 : fetal kidney cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-mH2A1 antibody [EPR9358] (ab137117)

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