Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)
![Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221572-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg "Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10085(B)] to Histone H3.3C
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Histone H3.3C antibody [EPR10085(B)]
See all Histone H3.3C primary antibodies -
Description
Rabbit monoclonal [EPR10085(B)] to Histone H3.3C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, testis and SH-SY5Y lysates; Human kidney and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10085(B) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)
Nuclear staining of Mouse collon using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221571-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear staining of Mouse kidney using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221570-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear astaining of Mouse liver using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221569-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear staining of Mouse testis using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221568-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear staining of Rat colon usign ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221567-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear staining of Rat Lung tissue using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-221565-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Nuclear staining of Rat Brain using ab150417 at 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157067-anti-histone-h33c-antibody-epr10085b-western-blot.jpg)
Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)All lanes : Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human testis lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157066-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157065-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157064-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Immunohistochemical analysis of paraffin embedded normal Human ovary tissue using ab150417 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157063-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab150417 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-157062-anti-histone-h33c-antibody-epr10085b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)Immunohistochemical analysis of paraffin embedded Human Thyroid gland tissue using ab150417 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)](https://www.abcam.com/ps/products/150/ab150417/Images/ab150417-14-benefits-of-recombinant-antibodies.png)
Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)