Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10085(B)] to Histone H3.3C
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H3.3C antibody [EPR10085(B)]
See all Histone H3.3C primary antibodies
Description

Rabbit monoclonal [EPR10085(B)] to Histone H3.3C
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, testis and SH-SY5Y lysates; Human kidney and tonsil tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10085(B)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Mouse collon using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Mouse kidney using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear astaining of Mouse liver using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Mouse testis using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Rat colon usign ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Rat Lung tissue using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Nuclear staining of Rat Brain using ab150417 at 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

All lanes : Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human testis lysate
Lane 3 : SH-SY5Y lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Histone H3.3C with ab150417 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Immunohistochemical analysis of paraffin embedded normal Human ovary tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland tissue using ab150417 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Histone H3.3C antibody [EPR10085(B)] (ab150417)

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