HRP Anti-Histone H2B antibody (ab64039)
Key features and details
- HRP Rabbit polyclonal to Histone H2B
- Suitable for: WB
- Reacts with: Mouse, Rat, Recombinant fragment
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Histone H2B antibody
See all Histone H2B primary antibodies -
Description
HRP Rabbit polyclonal to Histone H2B -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H2B aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16101) -
Positive control
- This antibody gave a positive signal in NIH3T3 and PC12 whole cell lysates, and when tested against Histone H2B recombinant protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - HRP Anti-Histone H2B antibody (ab64039)All lanes : HRP Anti-Histone H2B antibody (ab64039) at 1/40000 dilution
Lane 1 : Histone H1 Recombinant Protein
Lane 2 : Histone H2A Recombinant Protein
Lane 3 : Histone H2B Recombinant Protein
Lane 4 : Histone H3.1 Recombinant Protein
Lane 5 : Histone H4 Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Western blot - HRP Anti-Histone H2B antibody (ab64039)All lanes : HRP Anti-Histone H2B antibody (ab64039) at 1/40000 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
