Anti-gamma H2A.X (phospho S139) antibody (ab11174)
Key features and details
- Rabbit polyclonal to gamma H2A.X (phospho S139)
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-gamma H2A.X (phospho S139) antibody
See all gamma H2A.X primary antibodies -
Description
Rabbit polyclonal to gamma H2A.X (phospho S139) -
Host species
Rabbit -
Specificity
Using IF, this antibody was shown to bind to a non-nuclear location in Hela cells. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF MouseHumanIHC-P HumanWB Human
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Immunogen
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
Samples:
Nuclear extract (50µ UT = untreated
Ub = ubiquitylated
NCS = neocarzinostatin - 200 ng/ml, 30 minSamples: Nuclear extract (50
µg) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: ab11174 used at 0.1 mcg/ml. Detection: Chemiluminescence with 30 second exposure. UT = untreated
Ub = ubiquitylated
NCS = neocarzinostatin - 200 ng/ml, 30 min -
Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174)Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) labelling H2A.X (phospho S139 with ab11174 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (ab11174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling gamma H2A.X (phospho S139) with ab11174 at 1/5000 (0.2µg/ml). Dectection: DAB.
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Flow Cytometry - Anti-gamma H2A.X (phospho S139) antibody (ab11174)ab11174 at 0.5ug staining gamma H2A.X in human Jurkat cell line by flow cytometery. Cells were treated with 5ug/ml etopside for 3 hours, fixed in 1.5% paraformaldehyde, and permeabilized in 90% methanol. A FITC-conjugated rabbit polyclonal was used as secondary (in 150ul reaction). The black line indicates cells treated with etopside and anti KLH antibody, the red line repersents untreated cells and anti gamma H2A.X and blue line indicates etopside treated anti gamma H2A.X respectively.
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Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) This image is courtesy of an anonymous AbreviewAll lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 1/5000 dilution
Lane 1 : HeLa nuclear lysate - untreated
Lane 2 : HeLa nuclear lysate - IR treated
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174)Detection of gamma-H2AX by Immunofluorescence. Samples: Wildtype (H2AX +/+) or H2AX knockout (H2AX -/-) mouse embryonic fibroblasts. Antibody: Affinity purified ab11174 used at 2ug/ml. Detection: Rhodamine red labelled goat anti-rabbit IgG. -
Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174) This image is courtesy of an anonymous Abreviewab11174 at 1/1000 staining human HeLa cells by ICC/IF. These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.
