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Epigenetics and Nuclear Signaling Histones Variants

Anti-HMGN2 antibody [EPR7091] (ab124997)

Price and availability

247 929 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-HMGN2 antibody [EPR7091] (ab124997)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7091] to HMGN2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-HMGN2 antibody [EPR7091]
    See all HMGN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7091] to HMGN2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human HMGN2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HL60, K562, HeLa, SH-SY5Y and Jurkat cell lysates IHC-P: Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7091
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants

Images

  • Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
    Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
    All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution (Purified)

    Lane 1 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 9 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997)

    ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
    Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
    All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 9 kDa



    All cell lysates were prepared in 1%SDS Hot lysis method.

  • Anti-HMGN2 antibody [EPR7091] (ab124997)
    Anti-HMGN2 antibody [EPR7091] (ab124997)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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