Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab206898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EP854(2)Y] to gamma H2A.X (phospho S139)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Overview
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Product name
Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]
See all gamma H2A.X primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EP854(2)Y] to gamma H2A.X (phospho S139) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP854(2)Y -
Isotype
IgG -
Research areas
Images
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ab206898 staining Histone H2A.X (phospho S139) in Jurkat cells. The cells were incubated with 25µM Etoposide for 5 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab206898 at 1/100 dilution (shown in red) and ab195887 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488) at 2µg/ml (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
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