Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655)
Key features and details
- Rabbit polyclonal to gamma H2A.X (phospho S139) - C-terminal
- Suitable for: WB, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-gamma H2A.X (phospho S139) antibody - C-terminal
See all gamma H2A.X primary antibodies -
Description
Rabbit polyclonal to gamma H2A.X (phospho S139) - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC - Wmt ZebrafishWB Zebrafish
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Immunogen
Synthetic peptide within Zebrafish Histone H2A.X (C terminal) (phospho S139). The exact sequence is proprietary.
Database link: Q7ZUY3 -
Positive control
- IHC-Wm: 24 hours post-fertilization (UV-irradiated) and 2 days-post-fertilization zebrafish embryos. WB: UVC treated 3dpf zebrafish embryos extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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IHC - Wholemount - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655)
24 hours-post-fertilization, UV-irradiated zebrafish embryo stained for Histone H2A.X (phospho S139) with ab228655 (green) at 1/100 dilution in immunohistochemical wholemount analysis.
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Western blot - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655)All lanes : Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655) at 1/1000 dilution
Lane 1 : 3dpf zebrafish embryo whole cell extract
Lane 2 : 3dpf zebrafish embryos treated with 8000 J/m2 UVC, whole cell extract
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 15 kDa15% SDS-PAGE
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IHC - Wholemount - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655)2 days-post-fertilization zebrafish embryo stained for Histone H2A.X (phospho S139) with ab228655 at a 1:100 dilution in immunohistochemical wholemount analysis.
